Fig. 2

LncRNA-TBP inhibits myoblast proliferation but promotes myogenic differentiation. A Relative LncRNA-TBP expression with LncRNA-TBP overexpression in vitro (n = 6). B Relative mRNA levels of several cell cycle genes with overexpression of LncRNA-TBP (n = 6). C The proliferation of transfected CPMs was assessed by 5-ethynyl-2’-deoxyuridine (EdU) incorporation (n = 3). D The proliferation rate of myoblasts after the overexpression of LncRNA-TBP (n = 8). E CCK-8 assays were performed in CPMs with LncRNA-TBP overexpression (n = 6). F Cell cycle analysis of myoblasts after the overexpression of LncRNA-TBP (n = 4). G–I MyHC immunostaining (n = 3) (G), myotube area (%) (n = 8) (H) and myoblast fusion index (n = 8) (I) of CPMs transduced with LncRNA-TBP overexpression. Cells were differentiated for 72 h after transfection. J and K Relative mRNA (n = 6) (J) and protein (n = 3) (K) expression levels of myoblast differentiation marker genes with LncRNA-TBP overexpression. In panel H, the numbers shown below the bands were folds of band intensities relative to control. Band intensities were quantified by ImageJ and normalized to GAPDH. Data are expressed as a fold-change relative to the control. Results are shown as mean ± SEM. In panels (A, B, D–F and H–J), the statistical significance of differences between means was assessed using an independent sample t-test. (*P < 0.05; **P < 0.01; N.S., no significant difference)