Fig. 1

Identification of LncRNA-TBP. A LncRNA-TBP expression of pectoralis major and soleus of 7-week-old Xinghua chickens by RNA-seq (n = 4). B Relative LncRNA-TBP expression of pectoralis major and soleus of 7-week-old Xinghua chickens by qRT-PCR (n = 4). C Results of LncRNA-TBP 5’ RACE and 3’ RACE. D Relative LncRNA-TBP expression in polyadenylated RNA and total RNA (n = 4). E Relative LncRNA-TBP expression during CPMs proliferation and differentiation (n = 3). F Tissues expression profiles of LncRNA-TBP. The horizontal axis and vertical axis indicate different tissues and their relative expression values, respectively (n = 3). G The distribution of LncRNA-TBP in the cytoplasm and nuclei of CPMs was determined by qRT-PCR. GAPDH and U6 serve as cytoplasmic and nuclear localization controls, respectively. H Western blot analysis of the coding ability of LncRNA-TBP. The potential ORFs of LncRNA-TBP were cloned into the pcDNA3.1-3xFlag-C vector. CPMs transfected with β-actin were used as a positive control (PC) and untransfected CPMs were used as a negative control (NC). Results are presented as mean ± SEM. In panels (A, B and D), the statistical significance of differences between means was assessed using independent sample t-test. (*P < 0.05; **P < 0.01)