Fig. 4
From: FGF12 is a novel component of the nucleolar NOLC1/TCOF1 ribosome biogenesis complex
Role of FGF12 in the nucleolar localization of NOLC1 and TCOF1 and the assembly of their complexes. A Fluorescence microscopy analysis of the nucleolar localization of FGF12, NOLC1 and TCOF1 upon silencing of individual proteins. U2OS-FGF12-mGFP-myc cells, transfected with siRNA targeting NOLC1, TCOF1, FGF12 or scramble siRNA (control), were fixed, permeabilizated and stained with anti-NOLC1 or anti-TCOF1 primary antibodies and Alexa Fluor 594-conjugated secondary antibody. Cell nuclei were labeled with NucBlue Live and cells were analyzed by fluorescence microscopy. The scale bar represents 20 μm. B Fluorescence images of in situ PLA using anti-FGF12, anti-NOLC1 and anti-TCOF1 antibodies in U2OS-FGF12-mGFP-myc cells upon NOLC1, TCOF1 (n = 3) or FGF2 (n = 2) silencing. Cell nuclei were labeled with NucBlue Live and cells were analyzed by fluorescence microscopy. The dashed line indicates the cell area. The scale bar represents 20 μm. Data shown in the graphs are PLA signal intensity in nuclei. Student’s t-test was applied for statistical analysis; ***p < 0.001