Fig. 2
From: FGF12 is a novel component of the nucleolar NOLC1/TCOF1 ribosome biogenesis complex
Interaction of FGF12 with nucleolar proteins. A Verification of the results of MS experiments. Recombinant FGF12-His was bound to Ni Sepharose and incubated with U2OS cells lysate. FGF12-bound proteins were eluted and analyzed by wester blotting using specific antibodies. B Co-localization of FGF12-mGFP-myc with identified partner protein. To analyze the co-localization of FGF12-mGFP-myc with NOLC1, TCOF1, BCLAF1, THRAP3, U2OS-FGF12-mGFP-myc cells were fixed, permeabilized and stained with appropriate primary antibodies and Alexa Fluor 594-conjugated anti-mouse secondary antibody. Cell nuclei were labeled with NucBlue Live and cells were analyzed by fluorescence microscopy. The scale bar represents 20 μm.