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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: PTEN overexpression and nuclear β-catenin stabilization promote morular differentiation through induction of epithelial–mesenchymal transition and cancer stem cell-like properties in endometrial carcinoma

Fig. 6

Relationship between EBP50, PTEN, and β-catenin in Em Ca with morules. A Upper: HE and IHC staining for the indicated proteins in Em Ca with morules. Morular lesions and membranous EBP50 immunoreactivity are indicated by arrows and arrowheads, respectively. Original magnification, × 200. Lower: IHC score for the indicated molecules in Em Ca with morules. The data shown are as means ± SDs. Me-EBP50, membranous EBP50; Cyt-EBP50, cytoplasmic EBP50. B Left: immunofluorescence for EBP50 and PTEN. A lack of coimmunolocalization for EBP50 and PTEN in morules (indicated by asterisks) and the surrounding carcinoma (Sur Ca, indicated by an arrow) in Em Ca with morules. Original magnification, × 200. Right: western blotting (WB) with anti-EBP50 (upper panel) and anti-PTEN antibodies (lower panel) after immunoprecipitation (IP) with the indicated antibodies using H6-PTEN cell lysates. Input represents 5% of the total cell extract. Normal rabbit IgG was used as a negative control. C Left: immunofluorescence for EBP50 and β-catenin. Note the coimmunolocalization of EBP50 and β-catenin in the apical membrane of Sur Ca cells (indicated by arrows) but not morules (indicated by asterisks). Original magnification, × 200. Right: proteins bound to the beads were analyzed following by western blot analysis for β-catenin in Hec251 cells (upper). Detection of GST-bound EBP50 protein by Coomassie Brilliant Blue (CBB) (lower)

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Cell Communication and Signaling

ISSN: 1478-811X