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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: PTEN overexpression and nuclear β-catenin stabilization promote morular differentiation through induction of epithelial–mesenchymal transition and cancer stem cell-like properties in endometrial carcinoma

Fig. 3

Relationship between PTEN overexpression and cyclin D2 expression. A Unsupervised hierarchical clustering of mRNA expression detected by RNA-seq assay in H6-PTEN and mock cells. The level of expression of each mRNA is colored; red, black, and green indicated high (> 4), neutral (1–4), and low (< 1), respectively. Major clusters are shown as groups I to IX. Cyclin D2 (CCND2) is included in group VI. B Analysis of endogenous cyclin D2 mRNA expression by conventional (left upper and lower) and real time RT-PCR assay (right) in H6-PTEN and mock cells. The values of endogenous cyclin D2 mRNA expression detected by conventional RT-PCR assay were normalization to GAPDH. The fold changes in mRNA expression for both assays are presented as means ± SDs (left lower). C Western blot analysis for the indicated proteins in total lysates from H6-PTEN and mock cells. D Western blot analysis for the indicated proteins in total lysates from spheroid and differentiated Hec6 cells

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Cell Communication and Signaling

ISSN: 1478-811X