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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: PTEN overexpression and nuclear β-catenin stabilization promote morular differentiation through induction of epithelial–mesenchymal transition and cancer stem cell-like properties in endometrial carcinoma

Fig. 2

Relationship between PTEN overexpression, cell migration, and cancer stem cell-like features. A Upper: wound-healing assay with H6-PTEN and mock cells. A scratch ‘wound’ was introduced to the middle of wells containing cells grown to confluency, and phase contrast images were taken after 12 and 24 h. Lower: the values of wound areas in 0 h were set as 1. The fold wound areas are presented as means ± SDs. B Migration rate measured using a transwell assay. Upper: the H6-PTEN and mock cells were seeded in a 24-well transwell plates and incubated for 24 h in medium without serum. Cells were stained with HE and counted using a light microscope. Lower: the numbers of migrated cells are presented as means ± SDs. C Western blot analysis for the indicated proteins in total lysates from H6-PTEN and mock cells. D Aldefluor analysis in H6-PTEN and mock cells. Note the R1 populations including the ALDHhigh population with cancer stem cell-like features. E Upper: phase-contrast photographs of spheroids formed by H6-PTEN and mock cells following 2 weeks of growth. Lower: the numbers of spheroids are presented as means ± SDs

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Cell Communication and Signaling

ISSN: 1478-811X