Fig. 2

HG-induced PTEC-derived exosomes contribute to MFT in MCs through miR-92a-1-5p. A The flow chart of miR-92a-1-5p functional assay. B-F miR-92a-1-5p expression in renal PTECs (RPTECs) of a normal individual and a type 2 diabetic patient, HK-2 cells treated with NG and HG conditions for 48 h, exosomes derived from human RPTECs and HK-2 cells treated with HG for 48 h, and MMCs treated with HG-induced HK-2 cell-derived exosomes for 24 h. G-J miR-92a-1-5p levels in PTECs, PTEC-derived exosomes and MCs isolated from db/m mice and db/db mice and MCs isolated from C57B6 mice treated with HK-2 cell–derived from exosomes under NG and HG assessed by quantitative real-time PCR. K MMCs were transfected with either miR-92a-1-5p mimic (100 nM) or mimic control (miR-NC, 100 nM). After 24 h post-transfection, cells were cultured under NG condition for 24 h. L MMCs were transfected with miR-92a-1-5p inhibitor (50 nM) or control inhibitor (anti-miR-NC, 50 nM) for 24 h and then incubated with exosomes derived from HK-2 cells under NG or HG condition for another 48 h. N-cadherin, vimentin, collagen I and E-cadherin expressions were assessed using western blotting. The bar graph represents the mean ± S.E.M. of at least three independent experiments. RPM, read per million. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t-test or ANOVA followed by the post hoc test adjusted with Tukey’s correction