Fig. 5
From: UBE2O ubiquitinates PTRF/CAVIN1 and inhibits the secretion of exosome-related PTRF/CAVIN1
UBE2O limits caveolae formation. A Flag-UBE2O was transfected into HeLa cells. After 40 h transfection, HeLa cells were immunostained with an anti-Flag antibody and anti-PTRF antibody. B, C UBE2O-Myc was transfected into Flag-PTRF stable HeLa cell line. Caveolae structures in HeLa cells were identified by transmission electron microscopy (TEM) scanning. B Red arrowhead: caveolae, Scale bar: 200 nm. The number of caveolae per micrometer of the plasma membrane was quantitated through 13 cells analysis. C P values were calculated with Student’s t-test. ***P \(<\) 0.001