Fig. 3

The CR2 domain in UBE2O interacts with SDPR. A Flag-PTRF or Flag-SDPR expression plasmid was infected into HeLa cells. After cell lysates were prepared, Flag-PTRF or Flag-SDPR in cell lysates was immunoprecipitated with Flag M2 beads. PTRF-associated endogenous UBE2O or SDPR-associated endogenous UBE2O was detected by IB. B HeLa cells were transiently transfected with Flag-UBE2O vector. Flag-UBE2O-associated endogenous SDPR was detected by IP and IB. C Wild-type SDPR and SDPR deletion mutants D1 and D2 were shown as schematic representations. D UBE2O-Myc and Flag-SDPR or Flag-SDPR deletion constructs (D1–D2) vectors were transfected into HEK293T cells. After 40 h transfection, cells were harvested for IP and IB analyses. E Flag-SDPR and five UBE2O deletion constructs (D1–D5) were expressed in HEK293T cells. After cell lysates were precipitated with Flag M2 beads, immunoprecipitates and the total cell lysates were immunoblotted with the indicated antibody as shown