Fig. 6

Regulation of IARS in PLEKs and NHEKs related to STAT3. A A Western blot analysis was performed to measure the expression of IARS, STAT3 and pSTAT3(Tyr 705) in PLEKs treated with various concentrations of mupirocin for 24 h. β-actin was used as loading control. B A Western blot analysis was performed to measure the expression of STAT3 and pSTAT3(Tyr 705) in PLEKs treated with 100 μM mupirocin for different minutes. C A Western blot analysis was performed to measure the expression of IARS, STAT3 and pSTAT3(Tyr 705) in PLEKs treated with clobetasol(1 and 10 μM) and calcipotriol (20 and 200 nM) for 48 h. D A Western blot analysis was performed to measure the expression of IARS, STAT3 and pSTAT3(Tyr 705) in NHEKs treated with IL-17A(200 ng/ml),TNFα(10 ng/ml),IL-17A(200 ng/ml) combine TNFα (10 ng/ml), IL-23(100 ng/ml) and IL-6(100 ng/ml) for 24 h. Representatives immunofluorescence images (E) and quantification analysis (F) of IARS in PLEKs treated with various concentrations of mupirocin for 24 h. Scale Bar = 100 μm. (n = 5 ± SEM). **P < 0.01 versus 0 μM