Fig. 5

Mupirocin inhibited cell proliferation while promoted apoptosis in PLEKs. PLEKs were treated with various concentrations of mupirocin. A Cell proliferation ability in PLEKs treated for 48 h was evaluated using EdU staining (right). Red is EdU-555 staining. Scale Bar = 50 μm. The percentage viability (live cell count/total cell count) was calculated and expressed as mean ± SEM in five representative fields for each group (left). *P < 0.05, **P < 0.01 versus 0 μM. B Comparison of cell proliferation in PLEKs treated for 48 h by CCK-8 assay. (n = 6 ± SEM). *P < 0.05, **P < 0.01 versus 0 μM. C Western blotting analysis of Bax, Caspase3 and cleaved Caspase-3 protein expression in PLEKs treated for 24 h. β-actin was used as the loading control. D Apoptotic cell-death was measured by annexin V-FITC/PI staining in PLEKs treated for 48 h. E The graph represents the mean ± SEM of the percent of apoptotic cells in the flow cytometry results. (n = 3). *P < 0.05, versus 0 μM