Fig. 4

Mupirocin altered immunocytes compositions in IMQ-treated mice. Representative immunofluorescence images (right) and quantification analysis (left) of CD4(A), CD8(B), and Ly6G(C) on the skin of the vehicle-treated, MUP + IMQ-treated and IMQ-treated mice. Scale Bar in (A) and (C) is 100 μm. Scale Bar in (B) is 50 μm. (n = 5 ± SEM), *P < 0.05 versus vehicle. Representative flow results of MHCII⁺CD207⁺cell (D) and percentage analysis (J) in the epidermis. Representative flow results of CD3⁺CD8⁺T cell (E), Ly6G⁺ neutrophils(F), IL-17⁺ γδT cell (G), MHCII⁺F4/80⁻CD11c⁺ DC cell(H)and MHCII⁺F4/80⁺ macrophage (I) and quantification analysis (J) in the LN. (n = 3 ± SEM). *P < 0.05, ** P < 0.01 versus IMQ. Flow cytometry gating strategies are showed in the Additional file 2