Fig. 5

Act1/TRAF6/p38 MAPK pathway contributes to IL-17 mediated ferroptosis in HALI. a Immunofluorescence staining of lung tissues to detect the expression and location of GPX4 (red) and IL-17 (green) in each group. Scar bar = 20 μM. Nuclei were stained with DAPI (blue). b–k IL-6, TGF-β1, IL-17A, IL-17RA, Act1, TRAF6, p38 MAPK, p-p38 MAPK, GPX4 protein levels in each group were demonstrated by western blotting. l–m IL-17RA, Act1, TRAF6, p38 MAPK, p-p38 MAPK, GPX4 protein levels in Sal + Hyperoxia and Sal + Hyperoxia + IL-17A groups were demonstrated by western blotting. Data are shown as mean ± SEM. n = 3 per group. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^#P < 0.05, ^##P < 0.01, ^###P < 0,001, ^&P < 0.05, ^&&P < 0.01 between the groups. ^*Compared with the sham group. ^#Compared with the hyperoxia group. ^&Compared with the Sal + Hyperoxia group