Fig. 4

Inhibition of ferroptosis via IL-17A contributes to Sal-alleviated HALI. KM mice were divided into four groups: sham, Sal + hyperoxia, hyperoxia, Y-320 + hyperoxia. Mice were exposed to over 90% oxygen for 24 h, in the first three days before exposure to the hyperoxia, the Sal group mice were treated with Sal (100 mg/Kg), and the Y-320 group mice were treated with Y-320 (2 mg/Kg) once orally every day, while the rest of groups were given equal isotonic saline. a Representative H&E- and Masson trichrome-stained lung sections after different treatments. Morphology was examined using light microscopy. Scar bar = 20 μM. b Lung injury score was tested in each group. c The lung wet/dry weight ratio was analyzed in each group as indicated. d The expression and location of IL-17RA were detected by immunohistochemistry in each group. Morphology was examined using light microscopy. Scar bar = 20 μM. e The MDA level in each group was measured by MDA assay. f Endogenous levels of Fe²⁺ of the lung tissue in various groups were measured with an iron assay. g Transmission electron microscopy images of lung tissues in each group. The white arrow indicates lamellar body. The black arrows indicate mitochondria. Scale bars: 5 μM; 1 μM. Data are shown as mean ± SEM. n = 3 per group. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^#P < 0.05, ^##P < 0.01, ^###P < 0,001 between the groups. ^*Compared with the sham group. ^#Compared with the hyperoxia group