Fig. 3

Inflammation in HALI is associated with ferroptosis. a PPI network of IL-17A and GPX4. b–e Mice were exposed to over 90% oxygen for 12, 24, 48, 72 h respectively. Sham mice were included as controls. The protein expression levels of IL-6, IL-17A and GPX4 of lung tissues were analyzed by western blotting. f Endogenous levels of Fe²⁺ of the lung tissue in different groups were measured with an iron assay. Data are shown as mean ± SEM. n = 3 per group. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. ^*Compared with the sham group