Fig. 6

Establishment of in vitro system models based on tumor-derived organoids and culture enriched with cancer-associated fibroblasts (eCAFs) to validate the expression profile of ligand and receptor genes associated with the PI3K-AKT signaling pathway in ovarian cancer. Gene expression of CAFs ligands A and epithelial cells receptors (B) for these ligands. Genes were ordered based on a marker selection (signal to noise) that highlights the differences in the expression profile (normalized counts) of eCAFs A or normal tissues B compared to the other three conditions. C Top 50 highly variable genes from the PI3K-AKT signaling pathway across all samples. Genes were ordered based on a marker selection (signal to noise) that highlights the differences in the expression profile (normalized counts) between normal tissue and malignant cells. D Expression profile (normalized counts) of ligands and receptors of the PI3K-AKT signaling pathway across all samples. Rows (for ligand or receptor genes) were ordered based on a marker selection (signal to noise) that highlights the differences in the expression profile (normalized counts) of normal tissues when compared to the other three conditions