Fig. 5
HIF-1/2α knockdown reverses the JAK1 knockdown–induced growth and migration of EC cells. A Western blotting of the indicated proteins in WCLs from KLE cells expressing si ctrl, HIF-1α-siR, or HIF-2α-siR. The cells were incubated under hypoxia (2% O2). B CCK-8 cell proliferation analysis of KLE cells expressing NC (negative control), JAK1-sh1, JAK1-sh1 + HIF-1α-siR, or JAK1-sh1 + HIF-2α-siR. The cells were incubated under hypoxia (2% O2). Data are shown as the mean ± SD (n = 3). C Colony formation analysis of KLE cells expressing NC (negative control), JAK1-sh1, JAK1-sh1 + HIF-1α-siR, or JAK1-sh1 + HIF-2α-siR. The cells were incubated under hypoxia (2% O2). Data are shown as the mean ± SD (n = 3). Scale bar, 100 μm. D Transwell migration analysis of IKLE cells expressing NC (negative control), JAK1-sh1, JAK1-sh1 + HIF-1α-siR, or JAK1-sh1 + HIF-2α-siR. The cells were incubated under hypoxia (2% O2). Data are shown as the mean ± SD (n = 3). Scale bar, 100 μm