Fig. 5
Efflux capacity of BRAFV600E mutant MaMel cells. A Representative scatter plots of the side population (SP) assay detecting the efflux of DyeCycle Violet stain (DCV) in MaMel cells. The polygonal gates indicate the SP subsets (DCV-) for each cell line. B Quantification of the percentage of SP cells. C Heatmap displaying the normalized expression (z-score) of genes encoding for ABC transporter family members. D Representative scatter plots of the SP assay for MaMel21 after inhibition of ABC transporters with verapamil, Ko143, or the combination, in comparison to DMSO control. The polygonal gates indicate the SP subsets for each condition. E Quantification of the percentage of SP cells. All bar plots show the mean ± SD of three independent experiments (n = 3). *p < 0.05, **p < 0.01, ****p < 0.0001