Fig. 6

Effect of PHLPP isoform specific silencing or over-expression on glucose uptake. Three days post-proliferation, PHLPP1 or PHLPP2 was silenced or over-expressed using isoform specific siRNA or over-expression plasmid respectively, as indicated. (A–D) N2A cells or (E–F) SHSY-5Y were differentiated in serum-free medium in the absence of (MF) or chronic presence of 100 nM insulin (MFI) for 3 days. Differentiated cells were serum starved for 2 h, followed by 100 nM insulin for 30 min. Uptake of 2-NBDG was then measured. Bar represents relative change in uptake of 2-NBDG. Experiments were executed three times and a representative result is shown. Data expressed are mean ± SE. ***P < 0.001, **P < 0.01, *P < 0.05 compared to lane 1, ^###P < 0.001, ^##P < 0.01, ^#P < 0.05 as compared to lane 2. A.U Arbitrary Units