Fig. 1

Expression of PHLPP1 and PHLPP2 under insulin resistant condition in neuronal cells. A–B N2A cells were differentiated in serum-free medium in the absence of (MF) or chronic presence of 100 nM insulin (MFI) for 3 days. Cells were lysed and subjected to western blotting, followed by probing with relevant primary antibodies. A Bar represents relative change in PHLPP1 probed with anti-PHLPP1 antibody. B Bar represents relative change in PHLPP2 probed with anti-PHLPP2 antibody. C–D SHSY-5Y cells were differentiated in serum-free medium in the absence of (MF) or chronic presence of 100 nM insulin (MFI) for 3 days. Cells were lysed and subjected to western blotting, followed by probing with relevant primary antibodies. C Bar represents relative change in PHLPP1 probed with anti-PHLPP1 antibody. D Bar represents relative change in PHLPP2 probed with anti-PHLPP2 antibody. E–F Mice whole brain was lysed, subjected to western blotting, followed by probing with relevant primary antibodies. E Bar represents relative change in PHLPP1 probed with anti-PHLPP1 antibody. F Bar represents relative change in PHLPP2 probed with anti-PHLPP2 antibody. GAPDH has been used as a loading control. Experiments were executed three times and a representative result is shown. Data expressed are mean ± SE. ***P < 0.001, **P < 0.01 compared to lane 1 IB Immunoblot