Fig. 3
From: The ERα-NRF2 signalling axis promotes bicalutamide resistance in prostate cancer
The mechanisms by which NRF2 is regulated by E2. A Western blot analysis of the expression of NRF2 in LNCaP abl and PC3 cells with ERα knockdown and in LNCaP cells with ERα overexpression. B qPCR analysis of the expression of NRF2 and its target genes (t test). C qPCR and Western blot analysis of the indicated molecules in LNCaP abl or PC3 cells with or without ERα knockdown after treatment with either DMSO or 10 nM E2 (one-way ANOVA). D qPCR analysis of the indicated molecules in LNCaP abl or PC3 cells treated with or without TAM after treatment with either DMSO or 10 nM E2 (one-way ANOVA). E ChIP assay showing that ERα binds directly to the ERE in the NRF2 promoter region. F ERα transcriptional activity in LNCaP and LNCaP abl cells after different treatments (one-way ANOVA). All studies were repeated at least three times; the values are presented as the means ± SEMs; *P < 0.05, **P < 0.01, ***P < 0.001