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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Interplay of the transcription factor MRTF-A and matrix stiffness controls mammary acinar structure and protrusion formation

Fig. 3

Implication of the MRTF-SRF pathway in stiffness-dependent acini morphogenesis. A Relative MRTF/SRF luciferase reporter activity in transiently transfected MCF10A seeded on Matrigel (MG) or collagen I (COLI) and analysed at day 3 of acini morphogenesis (n = 4). B, C Effects of the Rho/MRTF/SRF pathway inhibitor CCG203971 (20 μM, day 4–8) on acini diameter and average percentage of protrusion-positive acini at day 8. Eleven or more acini were analysed per condition in 3 biologically independent replicates. B Individually measured acini diameter (diamond) and mean ± SEM (horizontal black line ± whiskers). C Percentage of protrusion formation. Significance was tested using an unpaired Student’s t-test. *p ≤ 0.05. Error bars, SEM

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Cell Communication and Signaling

ISSN: 1478-811X