Fig. 3

Silencing RRP9 induces gemcitabine chemosensitivity in PC cells. A RRP9 mRNA levels in control cells and RRP-9-siRNA-treated (siRNA#1 and siRNA#2) cells. Data are expressed by mean ± SD, n = 3. B RRP9 protein levels in control cells and RRP-9-siRNA-treated (siRNA#1 and siRNA#2) cells. Data are denoted by mean ± SD, n = 3. C Cell viability was assessed in control and RRP9-silenced cells treated with increasing concentrations of gemcitabine. Data are given as mean ± SD, n = 50 μM. IC50 gemcitabine in cells that indicated. D Representative colony formation images (left) and quantification (right) are shown for control and RRP9-silenced cells treated with vehicle or gemcitabine (50 μM). Data are denoted by mean ± SD, n = 3. E Apoptosis was measured in control and RRP9-silenced cells treated with vehicle or gemcitabine (50 μM). FACS analysis (left) and quantification (right) of levels of apoptosis. Data are given as mean ± SD, n = 3. Note: *p < 0.05, **p < 0.01