Fig. 2
From: RRP9 promotes gemcitabine resistance in pancreatic cancer via activating AKT signaling pathway
RRP9 overexpression promotes chemoresistance to gemcitabine in PC cells. A RRP9 mRNA levels in control cells and RRP-9-overexpressing (RRP9-OE) cells. Data are given as mean ± SD, n = 3. B RRP9 protein levels in control cells and RRP-9-overexpressing (RRP9-OE) cells. Data are expressed by mean ± SD, n = 3. C Cell viability was assessed in control and RRP9-overexpressing cells treated with increasing concentrations of gemcitabine. Data are given as mean ± SD, n = 50 μM. IC50 of gemcitabine in the indicated cells. D Colony formation was measured in control and RRP9-overexpressing cells treated with vehicle or gemcitabine (50 μM). Representative colony formation images (left) and quantification (right) are shown. Data are presented as mean ± SD, n = 3. E Apoptosis was measured in control and RRP9-overexpressing cells treated with vehicle or gemcitabine (50 μM). FACS analysis (left) and quantification (right) are given. Data are expressed by mean ± SD, n = 3. Note: **p < 0.01, ***p < 0.001, ****p < 0.0001