Fig. 7

PGE₂ regulates IL-10 secretion level by increasing the NF-κB binding activity. A Plasma IL-10 was determined by ELISA in mice (n = 6); ^*P < 0.05 versus Con group; ^#P < 0.05 versus AMD group. B, C Effects of PGE₂ on the luciferase activity in the HEK293 cell line transiently transfected with PGL3 basic, PGL3 I1, PGL3 I2, PGL3 I3, and PGL3 I4 (n = 4); ^*P < 0.05 versus Basic group; ^**P < 0.01 versus Basic group; ^#P < 0.05 versus I1 group; ^@@P < 0.01 versus I2 group; ^$$P < 0.01 versus I3 group. D Nuclear and cytoplasmic proteins isolated from BMDMs. The NF-κB binding activity was detected by EMSA (n = 3). E–H P65-NF-κB protein was measured by western blotting using β-actin as the cytoplasmic control and Lamin b as the nuclear control. Representative blots are shown, with quantification (n = 3); ^*P < 0.05, ^**P < 0.01 versus Con group; ^#P < 0.05 versus H₂O₂ group. I, J The levels of phospho-ERK/ERK, phospho-JNK/JNK, and phospho-p38/p38 were determined by western blotting. Quantification of the western blot band intensity is shown in the respective right panels (n = 3); ^*P < 0.05