Fig. 5
From: PGE2 promotes macrophage recruitment and neovascularization in murine wet-type AMD models
EP1R/PKC signalling is involved in macrophages activation A Immunofluorescence staining of BMDMs with F4/80 (red) and nuclei (blue) is presented; scale bar: 50 μm. B–F The protein expression levels of EP1-4R measured by western blot analysis using β-actin as the internal control. Representative blots are shown, with quantification (n = 3 per group). G, H The mRNA levels of the M1 and M2-specific markers were measured by qRT-PCR in the BMDMs treated with 1 μM PGE2, 5 μM PGE2, 10 μM 17-PT-PGE2, 10 μM Butaprost, 10 μM Sulprostone, 10 μM Cay10598, and 20 ng/mL IL-4, respectively. I The quantitative PCR analysis of IL-10, Ym1, and MRC2 expression in the BMDMs pretreated with 5 μM H89, 5 μM LY294002, and 15 μM Bis 1 for 30 min respectively before being supplemented with 5 μM PGE2 for 24 h (n = 3). J Immunofluorescence staining of choroidal flat mounts from the mice with anti-EP1R and anti-CD206 mAbs; scale bar: 100 μm. K Representative fundus and OCT image of the eyes after the laser-induced CNV model. L Immunofluorescence staining of the flat mounts from the mice with anti-IB4 mAbs. M Quantification of the CNV lesion area in the choroidal flat mounts (n = 6); **P < 0.01 vs. Con; and #P < 0.05 vs. PGE2 5 μM; and @P < 0.05 vs. PGE2 5 μM