Fig. 1

The activation of macrophages plays a key role in CNV development. A, B The proportion of CD206( +) or CD80( +) cells among CD11b-gated monocytes derived from the laser-induced mice at 3 dpi and 7 dpi was detected by flow cytometric analyses. C, D Immunofluorescence staining of the choroidal sections C and flat mounts D from the laser-induced mice (7 days post-injury [dpi]) with anti-F4/80 (green), anti-CD206 (red) and anti-CD80 (red) mAbs. E The mRNA levels of the M1 and M2-specific markers were measured by qRT-PCR at 7 dpi (n = 6). F The mice were treated with an intravitreal injection of 2 μl/eye of 90 μg clodronate liposomes. The representative vascular leakage image of FFA at 7 dpi and 14 dpi. G The immunofluorescence staining of the RPE-choroid complex mounts from mice at 7 dpi and 14 dpi with anti-IB4 mAbs were presented; scale bar: 100 μm. H Quantification of the CNV lesion area in the choroidal flat mounts (n = 6). I Representative fundus and OCT image of the eyes after the laser-induced CNV model (7 dpi and 14 dpi). Scale bar: 100 μm C, D and G. ^*P < 0.05; ^**P < 0.01