Fig. 5

S1PR2 mediated NF-κB activation and inflammatory factor expression in pancreatic acinar cells. NF-κB expression in 266-6 cells (A); immunofluorescence staining of NF-κB in 266-6 cells (B); mRNA levels of the inflammatory factor IL-6 and TNF-α in 266-6 cells (C); cell proliferation in 266-6 cells (D); NF-κB levels in 266-6 cells, preincubated with or without JTE-013, and stimulated with TCA (100 µM) for 4 h (E); S1PR2 and NF-κB levels in adenovirus control and Ad-S1PR2-transduced 266-6 cells stimulated with TCA (100 µM) for 4 h (F); NF-κB levels in 266-6 cells, preincubated with or without JTE-013, Y27632, U0126, SB203590, and stimulated with TCA (100 µM) for 4 h (G). Data shown are means ± SEM. *p < 0.01, **p < 0.05, ***p < 0.001 compared with the control group. ##p < 0.05 compared with the TCA-treated alone