Fig. 5

Catalase-deficient mature adult mice induce lipofuscin accumulation through ROS. A Representative hematoxylin and eosin (H&E) staining of livers from WT and catalase-KO mice at the age of 9 W and 53 W, respectively. The livers of 53 W KO mice showed microvesicular steatosis (i.e., accumulation of small fat droplets) in the cytosol of hepatocytes (arrows) with a golden-brown pigment (arrowhead) known as lipofuscin. Scale bar represents 50 μm. B Liver samples from mice as mentioned in A were homogenized, and TG levels were analyzed. Bar graph represents mean ± SD (n = 3 experiments). *P < 0.05, WT 53 W versus KO 53 W. C Cryosectioned liver tissues from mice were stained with ORO. Scale bar represents 50 μm. D Representative fluorescence images of MEFs at P2 and P5 treated with 5 mM NAC, fixed, and immunostained with Lysotracker (red), LGALS1 (green), and DAPI (blue). Scale bar represents 5 μm