Fig. 4

Catalase-deficient mature adult mice induce alteration of autophagy through ROS. A MEFs from WT and catalase-KO mice at P2 and P5 levels were treated with 5 mM NAC, fixed, and immunostained with anti-LC3 (green),Lysotracker (red), yellow showed LC3 puncta co-localized to lysosme (arrow head). Scale bar represents 10 μm. Quantification of B LC3, C lysotracker and D co-localized cell analyzed from each experimental group as in A. The bar graph represents the mean ± SD (n = 3 experiments). *P < 0.05; **P < 0.0001. E Proteins were extracted from MEFs as in A. Immunoblot analysis was performed using whole-cell lysates with the indicated antibodies. F Protein was extracted from liver lysates from WT and catalase-KO mice at 9 W and 53 W. Immunoblot analysis was performed using whole-cell lysates with the indicated antibodies