Fig. 1

Catalase-deficient mice induce aging phenotype faster than WT mice. A Morphology and senescence-associated β-galactosidase staining of WT and catalase KO MEFs at passage 2 (P2) and passage 5 (P5) levels. Intensities of senescent cells at each passage, P2 and P5, in WT and KO MEFs. Positive intensities of β-galactosidase staining were measured compared to control using the ImageJ software. Scale bar represents 100 μm. B Proteins were extracted from MEFs at P2 and P5 level. Immunoblot analysis was performed using whole-cell lysates with indicated senescence-associated antibody. C, D quantified protein level of P21 and P16 normalized with actin. E Morphology of dorsal back hair of WT and KO mice at 53 W. F Senescence-associated β-galactosidase staining was performed in the liver section of 9 weeks (9 W) and 53 weeks (53 W) WT and catalase-KO mice. Intensities of senescent cells. Positive intensities of β-galactosidase staining were measured compared to control using the ImageJ software. Scale bar represents 100 μm. G Immunoblot analysis was performed from the liver sample from WT and catalase-KO mice at the age of 9 W and 53 W with indicated antibody. H, I quantified protein level of P21 and P16 normalized with actin. Bar graph represents mean ± SD (n = 3 experiments). *P < 0.05; **P < 0.0001; ns: non-significant