Fig. 5

Sex- and age-related differences in the insulin signaling in gastrocnemius muscle. a–c Protein extract (60 μg protein) was separated by SDS-PAGE and subjected to Western blot analysis. We made tissue pools of 15 rats for p20 tissues and 5 rats for adult tissues. Results are product of two independent experiments to p-S6K1 and three to p-Akt. The bars represent the mean ± S.E.M. **P ≤ 0.009, ***P ≤ 0.001, ****P < 0.0001 compared to their control group by two-way ANOVA with post hoc Tukey's test; ^##P ≤ 0.007 compared between sex and fasting vs fed state by two-way ANOVA with post hoc Tukey's test; d summary of sex-related differences in the IR mechanism of PI3K/Akt signaling pathway in gastrocnemius muscle of p20 rats during postprandial state. PAT: peripancreatic adipose tissue. Created with GraphPad Prism V 9.3.1 (471) and BioRender.com