Fig. 4

Sex- and age-related differences in the insulin signaling in pWAT. a–c Protein extract (100 μg protein) was separated by SDS-PAGE and subjected to Western blot analysis. We made tissue pools of 15 rats for p20 tissues and 5 rats for adult tissues. Results are product of three independent experiments to p-S6K1 and p-Akt and two to p-IRS1. The bars represent the mean ± S.E.M. *P < 0.05, **P < 0.005, ***P = 0.0009, ****P < 0.0001 compared to their control group by two-way ANOVA with post hoc Tukey's test; ^#P < 0.05 compared between sex and fasting vs fed state by two-way ANOVA with post hoc Tukey's test; d Summary of sex-related differences in the IR mechanism of PI3K/Akt signaling pathway in pWAT of p20 rats during postprandial state. Created with GraphPad Prism V 9.3.1 (471) and BioRender.com