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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Exosomal AP000439.2 from clear cell renal cell carcinoma induces M2 macrophage polarization to promote tumor progression through activation of STAT3

Fig. 5

Knockdown of AP000439.2 inhibits the M2 polarization of macrophage. The expression of AP000439.2 in 786-O cells (A) and Caki-1 cells (D) were knockdown by using siRNA and knockdown efficiency was detected by qRT-PCR. The expression of AP000439.2 in exosomes derived from 786-O cells (B) and Caki-1 cells (E) after knockdown of AP000439.2 detected by qRT-PCR. The mRNA expression level of TGF-β and IL-10 in macrophage was detected by qRT-PCR after co-cultured with exosome derived from 786-O cells (C) and Caki-1 cells (F) after knockdown AP000439.2, and NC. (G) The protein expression level of TGF-β and IL-10 in macrophage was detected by western blot after co-cultured with exosome derived from 786-O cells and Caki-1 cells after knockdown AP000439.2. Data were analyzed from three distinct biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001, Two-tailed Student’s t-test for two groups, one-way ANOVA following Tukey’s test for three groups

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Cell Communication and Signaling

ISSN: 1478-811X