Fig. 4

Overexpression of AP000439.2 promotes the M2 polarization of macrophage. A The relative expression levels of AP000439.2 in exosomes derived from ccRCC (786-O, Caki-1) and human normal kidney proximal tubular cell line HK-2 detected by qRT-PCR. B AP000439.2 expression in AP000439.2 transient overexpressed 786-O and Caki-1 cells detected by qRT-PCR. C The mRNA expression level of TGF-β and IL-10 in macrophage was detected by qRT-PCR after co-cultured with exosome. Exosomes were extracted from the cell culture of 786-O and Caki-1 overexpressed AP000439.2, and vector negative control (NC), respectively. D The protein expression level of TGF-β and IL-10 in macrophage was detected by western blot after co-cultured with exosome. Exosomes were extracted from the cell culture of 786-O and Caki-1 overexpressed AP000439.2, and NC, respectively. E M1 markers (CD86 and NOS2) and M2 markers (TGF-β and IL-10) were detected by western blot in M2 macrophages with AP000439.2 knockdown. Data were analyzed from three distinct biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001, Two-tailed Student’s t-test