Fig. 7

TRIM31 is required for lysosome maturation but not acidification. A LysoTracker Red staining was examined in sh-NC and TRIM31-deficient GES-1 cells that were either untreated or primed with Hp supernatant for 12 h. B To measure Lysosomal pH, sh-NC and TRIM31-deficient GES-1 cells primed with or without Hp supernatant for 12 h were treated with LysoSensor Yellow/Blue DND-160 dye and 440/540 emission was evaluated. C, D To assess Cathepsin B activity, live sh-NC and TRIM31-deficient GES-1 cells both treated with Hp supernatant were stained with the Cathepsin B Magic Red assay kit, and staining intensity was evaluated by a Confocal fluorescence microscope. E Immunoblot analysis of Cathepsin B and Cathepsin D in sh-NC and TRIM31-deficient GES-1 cells treated with Hp supernatant for 12 h. F Western blotting analysis of NLRP3, cleaved caspase-1, pro-caspase-1, and cleaved IL-1β in sh-NC and TRIM31-deficient GES-1 cells treated with or without rapamycin (100 ng/m) for 8 h. Results are expressed as means ± SD. NS, not significant, **P < 0.01, ***P < 0.001