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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: The E3 ubiquitin ligase TRIM31 attenuates NLRP3 inflammasome activation in Helicobacter pylori-associated gastritis by regulating ROS and autophagy

Fig. 5

TRIM31 knockdown aggravated the mitochondrial damage and enhanced the production of mitochondrial ROS in GES-1 cells. A The mitochondrial membrane potential was measured using MitoTracker Deep Red and MitoTracker Green staining followed by flow cytometry. The percentage of MitoTracker Deep Redlow was used to assess the proportion of depolarized mitochondria. B The mitochondrial structure was observed by transmission electron microscopy. C MitoSOX was used to measure the level of mitochondrial ROS by flow cytometry. D Immunoblot analysis of NLRP3, pro-caspase-1, cleaved caspase-1, and cleaved IL-1β in different groups. Cells were primed with Hp supernatant alone or stimulated with Hp supernatant and Mito-TEMPO (500uM). E IL-1β concentration in the culture supernatant of sh-NC and TRIM31-silenced GES-1 cells was determined by ELISA. Results are expressed as means ± SD. *P < 0.05,**P < 0.01, ***P < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X