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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: The E3 ubiquitin ligase TRIM31 attenuates NLRP3 inflammasome activation in Helicobacter pylori-associated gastritis by regulating ROS and autophagy

Fig. 4

TRIM31 suppressed the activation of NLRP3 inflammasome and the production of IL-1β in GES-1 cells. The NC-shRNA and TRM31-shRNA lentivirus were transfected into the GES-1 cells. A The mRNA levels of TRIM31 in the sh-NC group and the sh-TRIM31 group were measured by PCR. B The protein expression of TRIM31 in the sh-NC group and the sh-TRIM31 group was determined by western blotting. C The mRNA levels of NLRP3 and IL-1β in the sh-NC group, the sh-TRIM31 group, the sh-NC + Hp group (sh-NC GES-1 cells treated with Hp supernatant for 12 h), and the sh-TRIM31 + Hp group (TRIM31-deficiency GES-1 cells treated with Hp supernatant for 12 h) were detected. D The level of IL-1β in the GES-1 cell culture supernatant was analyzed by ELISA. E The protein expression of NLRP3, pro-caspase-1, cleaved caspase-1, and cleaved IL-1β was measured by western blotting. Results are expressed as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X