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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: MICAL2 contributes to gastric cancer cell migration via Cdc42-dependent activation of E-cadherin/β-catenin signaling pathway

Fig. 8

MICAL2 accelerates E-cadherin degradation via a Cdc42-dependent mechanism. A The activities of Cdc42 in SGC-7901 cells transfected with a MICAL2 expression plasmid and BGC-823 cells transfected with siRNA targeting MICAL2 (siMICAL2) were measured using pulldown assays. B MICAL2-depleted BGC-823 cells were transfected with a Cdc42-Q61L expression plasmid and total cellular proteins were extracted and assessed for E-cadherin levels by western blotting assays. C–E MICAL2-depleted cells were transfected with the Cdc42-Q61L expression plasmid and following which the cell migration rate (C), the E-cadherin degradation rate (D), and E-cadherin ubiquitination (E) were assayed. **P < 0.01, ***P < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X