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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: MICAL2 contributes to gastric cancer cell migration via Cdc42-dependent activation of E-cadherin/β-catenin signaling pathway

Fig. 7

MICAL2 regulates cell migration via the Wnt/β-catenin signaling pathway. A BGC-823 cells were transfected with control siRNA or siRNA targeting MICAL2 (siMICAL2) for 48 h. Subsequently, total protein extracts were assessed for β-catenin levels and distribution. B β-catenin levels were measured in cytoplasmic and nuclear extracts obtained from BGC-823 cells transfected with siMICAL2. GAPDH served as the cytoplasmic control and histone H3 as the nuclear control. C Representative immunofluorescence images of β-catenin staining in MICAL2-depleted BGC-823 cells. Scale bar, 10 μm. D Representative immunofluorescence images of MICAL2-depleted BGC-823 cells pre-treated with LiCl. Scale bar, 10 μm. E–G MICAL2-depleted BGC-823 cells were pre-treated with LiCl, after which β-catenin protein levels in cytoplasmic and nuclear extracts (E), the cell migration rate (F), and E-cadherin levels (G) were determined. **P < 0.01, ***P < 0.001

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Cell Communication and Signaling

ISSN: 1478-811X