Fig. 3

Docetaxel regulated tumor glycolysis and growth in Smad3-dependnet way. A PC-3M cells were serum starved for 24 h and then left untreated or treated with docetaxel for an additional 1 h. Levels of cytosolic and nuclear Smad3 were determined by western blotting. α-tubulin and histone 3 were used as internal controls for the cytosolic and nuclear fractions. B The total protein was collected from PC-3M cells treated with shikonin for 1 h and separated by SDS-PAGE to detect the phosphorylation level of Smad3 (Ser213). PC-3M cells were treated with or without docetaxel for 24 h, and following by transfection with or without Smad3 plasmid for further 48 h, the level of lactate production (C), glucose consumption (D), and cell viability (E) were measured according to instruction, F the total protein was collected and subject by SDS-PAGE to detect indicated proteins, the band intensity was quantified, data represented the mean ± s.d. of three independent experiments and analyzed by One-way ANOVA with multiple comparisons, followed by Bonferroni post hoc test for significance. **P < 0.01, ***P < 0.001