Fig. 1
Docetaxel suppressed cell proliferation. A PC-3M and PC-3M IEC8 cells were digested and reseeded in a 96-well plate, respectively, and treated with docetaxel (5 uM) in a time course. The cell viability of PC-3M and PC-3M IE8 cells was determined by CCK-8 assay. Data represented the mean ± s.d. of three independent experiments and were analyzed by the one sample t test for significance versus the control group. ***P < 0.001. B After digestion, PC-3M IE8 and PC-3M cells were reseeded in a density of 105 in 96-well plate overnight, the cells were treated with docetaxel in a various of concentration for 48 h. The cell viability was measured by CCK-8 assay. Data represented the mean ± s.d. of three independent experiments and were analyzed by the one sample t test for significance versus the control group. *P < 0.05, **P < 0.01, ***P < 0.001. C Western blotting was performed to analyze cell proliferation characterized by PCNA and Ki-67 expression in PC-3M IE8 and PC-3M cells treated with or without docetaxel (5 uM) for 48 h, α-tubulin served as internal control and the intensity of bands was quantified and analyzed by by t test for significance versus the control group, Data represented the mean ± s.d. of three independent experiments, **P < 0.01, ***P < 0.001