Fig. 8From: Intracellular cholesterol transport inhibition Impairs autophagy flux by decreasing autophagosome–lysosome fusionMinimum concentration of bafilomycin A1 does not induce ER stress. RPE1 cells were treated with different concentrations of either thapsigargin or bafilomycin A1 in a serum-starvation medium for 24 h. RNAs were extracted, and the expression of CHOP and TRB3 was analyzed using quantitative real-time polymerase chain reaction (qPCR). Bar graph represents the mean ± SD (n = 3 experiments). *P < 0.05, Student's t-testBack to article page