Fig. 5

U18666A and BafilomycinA1 are potent inhibitors of intracellular cholesterol transport. A RPE1 cells were treated with U18666A (2 μg), bafilomycin A1 (25 nM), or chloroquine (5 μM) in a serum-starvation medium for 4 h. Cells were stained with filipin: scale bar, 10 μm. Arrowheads represent filipin-laden lysosomes B RPE1 cells were treated with U18666A (2 μg), bafilomycin A1 (25 nM), or chloroquine (5 μM) in a serum-starvation medium for 24 h. B Total RNA was extracted, and the expression of HMGCR, HMGCS, and INSIG1 was analyzed using a quantitative real-time polymerase chain reaction (qPCR). The bar graph represents the mean ± SD (n = 3 experiments). *P < 0.05, Student's t-test