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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Apoptin mediates mitophagy and endogenous apoptosis by regulating the level of ROS in hepatocellular carcinoma

Fig. 7

Effect of NIX protein on apoptin-induced mitophagy in liver cancer cells. A After addition of the ROS inhibitor, NAC, to the cells infected with Ad-apoptin, the mitophagy related protein level was detected by Western blot assay. B Expressions of LC3, cleaved caspase-3, NIX, BNIP3, and Ki67 in the xenograft tumor tissues were detected by IHC. C Western blot assay analysis of the changes in the expression levels of apoptosis and autophagy related proteins in MCF-7/ADR cells after silencing NIX and BNIP3. D Expressions of LC3 and cleaved caspase-3 in the xenograft tumor tissues were detected by IHC after silencing NIX and BNIP3. E After silencing NIX and BNIP3, the inhibition rate of HepG-2 cells was detected by the CCK-8 assay. F Analysis of the co-localization of NIX and HSP60 by immunofluorescence staining. G Co-immunoprecipitation assay to analyze the interaction between LC3 and NIX. The scale bar equals 20 µm. Data are shown as mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001) when compared with controls

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Cell Communication and Signaling

ISSN: 1478-811X