Fig. 6
The role of ROS in apoptin-mediated autophagy and apoptosis. A, B Quantitative analysis of changes in ROS level by DHR staining. C After the addition of the ROS inhibitor NAC (10 mM) to the cells infected with Ad-apoptin, the inhibition rate of HepG-2 cells was detected using CCK-8 assay. D, E Quantitative analysis of changes in apoptosis was conducted by using the Annexin-V FITC/PI assay. F, I. Western blot analysis of apoptosis-related protein expressions. J After LTR staining, the changes in lysosomal fluorescence intensity were observed by fluorescence microscope. K–O Western blot analysis of the expressions of autophagy-related proteins. The scale bar equals 50 µm. Data were representative of three independent experiments (n = 3). The unpaired Student’s t-test was used. *P < 0.05, **p < 0.01, ***p < 0.001. (#p < 0.05, ##p < 0.01) when compared with Ad-apoptin