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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Apoptin mediates mitophagy and endogenous apoptosis by regulating the level of ROS in hepatocellular carcinoma

Fig. 5

Analysis of the relationship between apoptin-mediated autophagy and mitochondria. A–C Qualitative analysis of changes in mitochondrial membrane potential by TMRM staining. With the prolongation of time, the mitochondrial membrane potential of the cells in the apoptin group decreased significantly, and the decrease was more obvious after adding 3-MA. D, E Quantitative analysis of changes in mitochondrial membrane potential by TMRM staining. F Analysis of the co-localization of mitochondria and lysosomes by MTG and LTR staining. G Formation of autophagosomes and their type of contents observed under an electron microscope. H Analysis of the co-localization of LC3 and HSP60 by immunofluorescence staining. The scale bar equals 50 µm. Data were representative of three independent experiments (n = 3). The unpaired Student’s t-test was used. *p < 0.05, **p < 0.01, ***p < 0.001. (#p < 0.05, ##p < 0.01) when compared with Ad-apoptin

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Cell Communication and Signaling

ISSN: 1478-811X