Fig. 4

Analysis of the relationship between apoptin-mediated autophagy and apoptosis. A After LTR staining in HepG-2 cells, the changes in lysosomal fluorescence intensity were observed by fluorescence microscopy. B–E Western blot analysis of the expressions of autophagy-related proteins. F and J Apoptosis analysis using flow cytometry after Annexin-V FITC/PI staining. G–I and K Western blot analysis of the expressions of apoptosis-related proteins. The scale bar equals 50 µm. Data were representative of three independent experiments (n = 3). The unpaired Student’s t-test was used. *p < 0.05, **p < 0.01, ***p < 0.001. (^#p < 0.05, ^##p < 0.01) when compared with Ad-apoptin