Fig. 2

Netrin-1 induced the anti-apoptotic effect of REH cells. A The 450 nm absorbance of REH cells treated with exogenous recombinant netrin-1 in concentration ladder of 0,25,50,100 ng/ml in 3 days. (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001). B The expression of CDK4, PCNA, Bcl-2 and Bax in REH cells treated with exogenous netrin-1 in concentration ladder (0,25,50,100 ng/ml, 24 h) was detected by western blotting. The expression of Gapdh was applied as an internal control. C Flow cytometric analysis of the apoptotic ratio of REH cells treated with exogenous recombinant netrin-1 in concentration ladder of 0, 25, 50 and 100 ng/ml after 24 h. The early apoptotic cells were marked with positive Annexin V staining (APC-A) and negative 7-AAD staining. D Column graph of the early and late apoptosis ratio of REH cells treated with exogenous recombinant netrin-1 in concentration ladder of 0,25,50 and 100 ng/ml after 24 h detected by flow cytometric analysis (n = 3, ***P < 0.001)